Coding

Part:BBa_K3141000:Design

Designed by: Keyi Huang   Group: iGEM19_BSC_United   (2019-10-15)


spacer; increasing proinsulin secretion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original spacer of vector has to be cut down before this spacer BBa_K3141000 been introduced to the sequence.


Source

Reading abundant papers and other relevant researches for reference, after the thorough consideration of procedures like protein expression, double digestion and purification, we defined the amino acid sequence of this spacer as EEAEAKR.

References